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020 _a9781627034531
024 7 _a10.1007/978-1-62703-453-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP623
_b2013 EB
245 0 0 _aCirculating MicroRNAs :
_bMethods and Protocols
_cedited by Nobuyoshi Kosaka
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 270 páginas)
_b44 ilustraciones, 22 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1024
505 0 _aFunctional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research -- Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture -- Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells -- Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse -- Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo -- Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells -- Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction -- Isolation of Circulating MicroRNA Associated with RNA-binding Protein -- Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids -- Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR -- Direct Serum Assay for MicroRNA in Cancer Patients -- Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ -- Analysis of Circulating MicroRNA by Microarray in Liver Disease -- Isolation of Circulating MicroRNA in Saliva -- Purification of RNA From Milk Whey -- Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases -- Methods of MicroRNA Quantification in Urinary Sediment -- Circulating microRNA for the Identification of Forensically Relevant Body Fluids -- Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease -- A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma -- Nanopore Single-molecule Detection of Circulating microRNAs.
520 _aMicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9144940
_aARN
776 0 8 _iPrinted edition:
_z9781627034548
776 0 8 _iPrinted edition:
_z9781627034524
776 0 8 _iPrinted edition:
_z9781493962990
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-453-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eIG
_zSI