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020			_a9781592591510
024	7		_a10.1385/1592591515 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC732 _b2001 EB
245	0	0	_aHuman Airway Inflammation : _bSampling Techniques and Analytical Protocols _cedited by Duncan F. Rogers, Louise E. Donnelly
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XV, 440 páginas) _b103 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v56
505	0		_aAirway Inflammation and Remodeling in Asthma -- Airway Inflammation and Remodeling in Asthma -- Sample Collection -- Biopsy Techniques -- Bronchoalveolar Lavage (BAL) -- Nasal Secretions and Exudations -- Sample Collection -- Induced Sputum -- Induced Sputum -- Measurement of Exhaled Nitric Oxide and Carbon Monoxide -- Measurement of Exhaled Hydrocarbons -- Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide -- Cell Isolation and Culture -- Airway Epithelial Cells (Primaries vs Cell Lines) -- Isolation and Culture of Human Alveolar Type II Pneumocytes -- Pulmonary Artery Endothelial Cells -- Isolation and Culture of Human Airway Smooth Muscle Cells -- Isolation and Purification of Human Mast Cells and Basophils -- Neutrophils -- Macrophages -- Collection, Separation, and Activation of Human T Lymphocytes -- Eosinophils -- Gene Expression/Regulation -- Analysis of Gene Expression -- Analysis of Gene Expression -- The Technique of In Situ Hybridization -- Measurement of Airway Mucin Gene Expression -- Transcription Factors and Inflammatory Gene Regulation -- Mediators -- Inflammatory Mediators in Spontaneously Produced Sputum -- Flow Cytometry -- Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry -- Current In Vitro Models of Leukocyte Migration -- Tracing Intracellular Mediator Storage and Mobilization in Eosinophils -- Measurement of Metalloproteinases -- Immunocytochemistry and Immunohistochemistry -- The Measurement of Cysteinyl Leukotrienes in Urine -- Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid -- Quantitative Analysis of F2 Isoprostanes.
520			_aAirway inflammation is now recognized as central to the pathophysiology of several major respiratory conditions-the two most common being asthma and chronic obstructive pulmonary disease (COPD). In Human Airway Inflammation: Sampling Techniques and Analytical Protocols, experienced basic and clinical investigators describe a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of many of the major inflammatory markers, along with protocols for measuring the inflammatory mediators and enzymes released during lung inflammation. Each user-friendly method contains all the essential step-by-step elements the researcher must navigate from sample collection to analysis, and includes notes on how to avoid problems. Among the more advanced techniques are the measurement of exhaled hydrocarbons and F2 isoprostanes, granulocyte pharmacodynamics in whole blood measured by flow cytometry, and tracing mediator trafficking in eosinophils using confocal microscopy. Comprehensive and highly practical, the methods presented in Human Airway Inflammation: Sampling Techniques and Analytical Protocols provide today's basic and clinical researchers with all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs.
988			_aSpringer_Protocols_2001
650		7	_2embne _9158916 _aDiagnóstico molecular
650		7	_2embne _9140908 _aAsma
776	0	8	_iPrinted edition: _z9781489943729
776	0	8	_iPrinted edition: _z9781489943712
776	0	8	_iPrinted edition: _z9780896039230
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591515 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b09/2023 _dz _eIG _zSI