000			04452nam a22003975i 4500
999			_c392459 _d392459
001			392459
003			ES-MaUEC
005			20230922085550.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			110915s2011 xxu| o |||| 0|eng d
020			_a9781617793103
024	7		_a10.1007/978-1-61779-310-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC365 _b2011 EB
245	0	0	_aNeuropeptides : _bMethods and Protocols _cedited by Adalberto Merighi
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 416 páginas) _b149 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v789
505	0		_aWhat Are Neuropeptides? -- Neuropeptide Localization in Non Mammalian Vertebrates -- Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons -- Neuropeptide RNA Localization in Tissue Sections -- Intron-Specific Neuropeptide Probes -- Direct In Situ RT-PCR -- Laser Capture Microdissection and Quantitative-PCR Analysis -- Visualization of Peptide Secretory Vesicles in Living Nerve Cells -- Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy (FCS) of Cell-Penetrating Neuropeptides -- Analysis of Neuro-Immune Interactions by an In Vitro Co-Culture Approach -- Electrophysiology -- Localization of Neuropeptides by Radioimmunoassay -- Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics -- Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis -- Suppression Subtractive Hybridization -- Neuropeptide Microdialysis in Free-Moving Animals -- Antibody Microprobes for Detecting Neuropeptide Release -- Neuropeptidases -- Neuropeptide Autoantibodies Assay -- Intranasal Delivery of Neuropeptides -- Prodrug Design for Brain Delivery of Small- and Medium-Sized Neuropeptides -- Measurement of Phosphorothioate Oligodeoxynucleotide Antisense Transport Across the Blood-Brain Barrier -- Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection -- Recombinant Adeno-Associated Viral Vectors -- De-Orphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay.
520			_aSince the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy.  Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field.  The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.
988			_aSpringer_Protocols_2011
650		7	_2embne _9502304 _aNeuropéptidos
776	0	8	_iPrinted edition: _z9781617793097
776	0	8	_iPrinted edition: _z9781617793110
776	0	8	_iPrinted edition: _z9781493962112
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-310-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI