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008			100301s1992 xxu| o |||| 0|eng d
020			_a9781592595006
024	7		_a10.1385/0896031993 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356.2 _b1992 EB
100	1		_aLongstaff, Alan _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688846
245	1	0	_aProtocols in Molecular Neurobiology _cby Alan Longstaff, Patricia Revest
250			_a1st edition 1992
264		1	_aTotowa, NJ _bHumana Press _c1992
300			_a1 recurso en línea (XVII, 373 páginas) _b68 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v13
505	0		_aSeparation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis -- The Isolation of Genomic DNA from Invertebrates -- Analysis of Mitochondrial DNA Mutations -- The Design and Use of Oligonucleotides -- The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences -- Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization -- Generation of Isoform-Specific Antisera from Cloned cDNAs -- Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System -- Mapping of Trans-Acting Factor Binding in the Nervous System -- In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes -- In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe -- The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice -- Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA-Injected Xenopus Oocytes -- Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues -- Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes -- Purification and Structure of L-Type Calcium Channels -- Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum -- Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing -- Voltage-Gated Ion Channels -- An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins.
520			_aNeurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literatu- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appre- ate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the inf- mation necessary to reproduce the technique and its results in any appropriately equipped laboratory.
988			_aSpringer_Protocols_1992
650		7	_2embne _9139103 _aBiología molecular
650		7	_2embne _9139039 _aNeurobiología
700	1		_aRevest, Patricia _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688847
776	0	8	_iPrinted edition: _z9781489940193
776	0	8	_iPrinted edition: _z9781489940186
776	0	8	_iPrinted edition: _z9780896031999
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896031993 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI