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008			130618s2013 xxu| o |||| 0|eng d
020			_a9781627034937
024	7		_a10.1007/978-1-62703-493-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR188.8 _b2013 EB
245	0	0	_aTransplantation Immunology : _bMethods and Protocols _cedited by Andrea A. Zachary, Mary S. Leffell
250			_a2nd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XIII, 411 páginas) _b49 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1034
505	0		_aAlloreactivity -- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of Injury -- Cell Mediated Rejection -- Transplantation Tolerance -- Composite Tissue Transplantation -- Transplantation of the Sensitized Patient: Histocompatibility Testing -- Thoracic Organ Transplantation: Laboratory Methods -- HLA Typing by Sequence Specific Primers -- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing -- Next-Generation HLA Sequencing using the 454 GS FLX System -- Chimerism Testing by Quantitative PCR  Using Indel Markers -- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR) -- Complement-dependent Cytotoxicity Crossmatch -- Lymphocyte Crossmatching by Flow Cytometry -- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies -- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens -- Tetramer Staining for the Detection of HLA-specific B cells -- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood -- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) -- Cylex  ImmuKnow Cell Function Assay -- Detection of Intracellular Cytokines -- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients -- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA -- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing.
520			_aAfter decades of research in clinical transplantation,  new techniques have been developed that permit a further understanding of the  immune mechanisms underlying immune recognition of allografts and a more accurate and  thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology.  The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research.  Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms,  as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.
988			_aSpringer_Protocols_2013
650		7	_2embne _9336019 _aTrasplante de órganos _xAspectos inmunológicos
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781627034944
776	0	8	_iPrinted edition: _z9781627034920
776	0	8	_iPrinted edition: _z9781493960156
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-493-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI