000 05002nam a22003975i 4500
999 _c392453
_d392453
001 392453
003 ES-MaUEC
005 20230916165323.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 100301s2008 xxu| o |||| 0|eng d
020 _a9781597451338
024 7 _a10.1007/978-1-59745-133-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP357
_b2008 EB
245 0 0 _aNeural Stem Cells :
_bMethods and Protocols
_cedited by Leslie P. Weiner
250 _a2nd edition 2008
264 1 _aTotowa, NJ
_bHumana Press
_c2008
300 _a1 recurso en línea (XVI, 406 páginas)
_b53 ilustraciones, 2 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v438
505 0 _aDefinitions and Criteria for Stem Cells -- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard -- Isolation and Culture of NSCs -- Neural Differentiation of Human Embryonic Stem Cells -- Generating Neurons from Stem Cells -- Isolation of Neural Stem and Precursor Cells from Rodent Tissue -- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization -- Adult Neural Stem Cells -- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb -- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium -- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property -- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood -- Production of Neurospheres from CNS Tissue -- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells -- In Vitro Studies: A. Cellular Techniques -- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures -- Telomere Neurobiology -- Chromosomal Mosaicism in Neural Stem Cells -- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype -- In Vitro Migration Assays of Neural Stem Cells -- Measuring Apoptosis in Neural Stem Cells -- Markers of Adult Neural Stem Cells -- In Vitro Studies: B. Molecular Techniques -- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR -- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells -- Electroporation Strategies for Genetic Manipulation and Cell Labeling -- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches -- Studies for Transplantation -- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Immunocytochemical Analysis of Neuronal Differentiation -- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold -- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS -- Optimizing Stem Cell Grafting into the CNS -- Routes of Stem Cell Administration in the Adult Rodent.
520 _aAlthough there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.
988 _aSpringer_Protocols_2008
650 7 _2embne
_9144348
_aNeuronas
650 7 _2embne
_9160528
_aCélulas madre
776 0 8 _iPrinted edition:
_z9781617378041
776 0 8 _iPrinted edition:
_z9781588298461
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-133-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eIG
_zSI