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008			100301s2009 xxu| o |||| 0|eng d
020			_a9781597451291
024	7		_a10.1007/978-1-59745-129-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP517.C45 _b2009 EB
245	0	0	_aRedox-Mediated Signal Transduction : _bMethods and Protocols _cedited by John T. Hancock.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 234 páginas) _b48 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v476
505	0		_aThe Role of Redox in Signal Transduction -- The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemiluminescence -- Detection and Measurement of Reactive Oxygen Intermediates in Mitochondria and Cells -- Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Review -- Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges -- Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with Egf -- Tools to Investigate ROS Sensitive Signalling Proteins -- Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine Phosphatase 1B as an Example -- Methods for the Study of Redox-Mediated Changes in p53 Structure and Function -- Redox Regulation and Trapping Sulphenic Acid in the Peroxide Sensitive Human Mitochondrial Branched Chain Aminotransferase -- Detection of Carbonylated Proteins in 2-D SDS Page Separations -- Analysis of Global and Specific Changes in the Disulfide Proteome Using Redox 2D-PAGE -- Protein-Thiol Oxidation, From Single Proteins to Proteome-Wide Analyses -- Analysis of Redox Relationships in the Plant Cell Cycle: Determinations of Ascorbate, Glutathione and Poly (ADPribose) Polymerase (PARP) in Plant Cell Cultures -- Generation and Detection of S-Nitrosothiols.
520			_aOnce believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years. In Redox-Mediated Signal Transduction: Methods and Protocols, experienced researchers with backgrounds in both the plant and animal sciences contribute timely methods and techniques that can be used to study this important aspect of biology. Beginning with an overview and methods for measuring compounds that affect redox and the redox state of cells, the book continues with reviews of the use of GFP and its derivatives, methods to study the impact of changing redox on proteins, and methods to study the exact molecular changes that may underlie the mechanisms of action of altering redox, among other subjects. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include step-by-step, readily reproducible protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Redox-Mediated Signal Transduction: Methods and Protocols is an ideal reference for those who wish to enter this exciting area of research as well as for those who simply wish for a more thorough understanding of the dramatic impact of redox in the control of cellular function.
988			_aSpringer_Protocols_2009
650		7	_2embne _9689361 _aTransducción de la señal celular
776	0	8	_iPrinted edition: _z9781603279253
776	0	8	_iPrinted edition: _z9781617378027
776	0	8	_iPrinted edition: _z9781588298423
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-129-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI