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008			130919s2014 xxu| o |||| 0|eng d
020			_a9781607618478
024	7		_a10.1007/978-1-60761-847-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH224 _b2014 EB
245	0	0	_aConfocal Microscopy : _bMethods and Protocols _cedited by Stephen W. Paddock
250			_a2nd edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 381 páginas) _b130 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1075
505	0		_aReflecting on Confocal Microscopy: A Personal Perspective.-  Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques -- Confocal Microscopy on the Internet -- Using Photoshop with Images Created by a Confocal System -- Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy -- Low Magnification Confocal Microscopy of Tumor Angiogenesis -- Confocal Imaging of Butterfly Tissue -- Confocal Microscopy of Cardiac Myocytes -- Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction -- Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens -- Vital Imaging of Multicellular Spheroids -- Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos -- Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells -- Measurement in the Confocal Microscope -- A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung -- Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney -- Evaluating Confocal Microscopy System Performance.
520			_aConfocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques. .
988			_aSpringer_Protocols_2014
650		7	_2embne _9163357 _aMicroscopía confocal
776	0	8	_iPrinted edition: _z9781588293510
776	0	8	_iPrinted edition: _z9781607618485
776	0	8	_iPrinted edition: _z9781493950676
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-847-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI