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007			cr nn 008mamaa
008			150424s2015 xxu| o |||| 0|eng d
020			_a9781493926398
024	7		_a10.1007/978-1-4939-2639-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2015 EB
245	0	0	_aPlant Mitochondria : _bMethods and Protocols _cedited by James Whelan, Monika W. Murcha
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XXIII, 303 páginas) _b40 ilustraciones, 28 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1305
505	0		_aIsolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa -- Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5' End Maturation -- Mitochondrial Run-On Transcription Assay Using Biotin Labeling -- In Vitro RNA Uptake Studies in Plant Mitochondria -- In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria -- Plant Mitochondrial Proteomics -- Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites -- A Flowchart to Analyze Protease Activity in Plant Mitochondria -- Activity Measurements of Mitochondrial Enzymes in Native Gels -- Activity Assay for Plant Mitochondrial Enzymes -- Analysis of Type II NAD(P)H Dehydrogenases -- Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes -- Micro-Respiration Measurements in Plants -- Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation -- Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana -- Imaging and Analysis of Mitochondrial Dynamics in Living Cells -- Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors -- In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide -- Databases and Informatics Resources For Analysis of Plant Mitochondria -- Expression and Crystallization of the Plant Alternative Oxidase.
520			_aThe chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein, and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.
988			_aSpringer_Protocols_2015
650		7	_2embne _9147980 _aMitocondrias
776	0	8	_iPrinted edition: _z9781493926381
776	0	8	_iPrinted edition: _z9781493926404
776	0	8	_iPrinted edition: _z9781493949069
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2639-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI