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003			ES-MaUEC
005			20230916114135.0
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007			cr nn 008mamaa
008			100301s2008 xxu| o |||| 0|eng d
020			_a9781597451888
024	7		_a10.1007/978-1-59745-188-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH440.5 _b2008 EB
245	0	0	_aGenomics Protocols _cedited by Mike Starkey, Ramnath Elaswarapu
250			_a2nd edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XV, 425 páginas) _b115 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v439
505	0		_aWhole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield -- Scanning for DNA Variants by Denaturant Capillary Electrophoresis -- Identification of SNPs, or Mutations in Sequence Chromatograms -- BeadArray-Based Genotyping -- Microsatellite-Based Candidate Gene Linkage Analysis Studies -- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) -- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification -- Application of Microarrays for DNA Methylation Profiling -- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray -- Transcriptional Profiling of Small Samples in the Central Nervous System -- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays -- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations -- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry -- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease -- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis -- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags -- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics -- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line -- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression -- Coimmunoprecipitation and Proteomic Analyses -- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo -- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation -- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells -- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data -- Comparative Genomics-Based Prediction of Protein Function -- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing.
520			_aSince the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.
988			_aSpringer_Protocols_2008
650		7	_2embne _9676129 _aMapa genético
650		7	_2embne _9164326 _aGenómica
776	0	8	_iPrinted edition: _z9781617378188
776	0	8	_iPrinted edition: _z9781588298713
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-188-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI