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008			140724s2014 xxu| o |||| 0|eng d
020			_a9781493911424
024	7		_a10.1007/978-1-4939-1142-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2014 EB
245	0	0	_aStable Isotope Labeling by Amino Acids in Cell Culture (SILAC) : _bMethods and Protocols _cedited by Bettina Warscheid
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIV, 372 páginas) _b65 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1188
505	0		_aTwelve Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) -- Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture -- SILAC Labeling of Yeast for the Study of Membrane Protein Complexes -- Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry -- Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons -- SILAC and Alternatives in Studying Cellular Proteomes of Plants -- In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster -- Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse -- Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans -- SILAC-based Temporal Phosphoproteomics -- Global Ubiquitination Analysis by SILAC in Mammalian Cells -- Quantifying In Vivo, Site-specific Changes in Protein Methylation with SILAC -- Applying SILAC for the Differential Analysis of Protein Complexes -- Defining Dynamic Protein Interactions Using SILAC-based Quantitative Mass Spectrometry -- Identifying Nuclear Protein-Protein Interactions using GFP Affinity Purification and SILAC-based Quantitative Mass Spectrometry -- Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC -- Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling -- Protein Correlation Profiling-SILAC to Study Protein-Protein Interactions -- Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC -- Design and Application of Super-SILAC for Proteome Quantification -- Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies -- Analysis of Secreted Proteins using SILAC -- Identification of MicroRNA Targets by Pulsed SILAC -- MaxQuant for In-Depth Analysis of Large SILAC Datasets.
520			_aStable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.
988			_aSpringer_Protocols_2014
650		7	_2embne _9430647 _aProteómica
650		7	_2embne _9683244 _aIsótopos
776	0	8	_iPrinted edition: _z9781493911431
776	0	8	_iPrinted edition: _z9781493911417
776	0	8	_iPrinted edition: _z9781493952571
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1142-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI