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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592593163
024	7		_a10.1385/159259316X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		3	_aQR201.E82 _b2003 EB
245	0	0	_aE. coli : _bShiga Toxin Methods and Protocols _cedited by Dana Philpott, Frank Ebel
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 352 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v73
505	0		_aThe Medical Significance of Shiga Toxin-Producing Escherichia coli Infections -- Methods for Detection of STEC in Humans -- Serological Methods for the Detection of STEC Infections -- Detection and Characterization of STEC in Stool Samples Using PCR -- Molecular Typing Methods for STEC -- STEC in the Food Chain -- STEC as a Veterinary Problem -- Cellular Microbiology of STEC Infections -- Analysis of Pathogenicity Islands of STEC -- Generation of Isogenic Deletion Mutants of STEC -- Generation of Monoclonal Antibodies Against Secreted Proteins of STEC -- Microscopic Methods to Study STEC -- Detection and Characterization of EHEC-Hemolysin -- Shiga Toxin Receptor Glycolipid Binding -- Methods for the Purification of Shiga Toxin 1 -- Methods for the Identification of Host Receptors for Shiga Toxin -- Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport -- Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin -- Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways -- Interaction of Shiga Toxin with Endothelial Cells -- Shiga Toxin Interactions with the Intestinal Epithelium -- Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes -- Animal Models for STEC-Mediated Disease -- Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC -- Bovine Escherichia coli O157:H7 Infection Model.
520			_aShiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods. Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.
988			_aSpringer_Protocols_2003
650		7	_2embne _9672589 _aInfecciones por Escherichia coli
650		7	_2embne _9138717 _aMicroorganismos
776	0	8	_iPrinted edition: _z9781617372643
776	0	8	_iPrinted edition: _z9781489938169
776	0	8	_iPrinted edition: _z9780896039391
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259316X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b09/2023 _dz _eIG _zSI