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008			100301s1993 xxu| o |||| 0|eng d
020			_a9781592595075
024	7		_a10.1385/0896032817 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP625.O47 _b1993 EB
245	0	0	_aProtocols for Oligonucleotides and Analogs : _bSynthesis and Properties _cedited by Sudhir Agrawal
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (XIV, 502 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v20
505	0		_aA Brief History of Oligonucleotide Synthesis -- Oligonucleotide Synthesis -- Oligodeoxyribonucleotides Synthesis -- Oligodeoxynucleotide Synthesis -- Oligoribonucleotide Synthesis -- Synthesis of 2?-O-Alkyloligoribonucleotides -- An Improved Method for the Synthesis and Deprotection of Methylphosphonate Oligonucleotides -- Oligonucleoside Phosphorothioates -- Synthesis and Purification of Phosphorodithioate DNA -- Oligodeoxyribonucleotide Phosphotriesters -- Oligonucleoside Boranophosphate (Borane Phosphonate) -- Oligonucleotide Phosphorofluoridates and Fluoridites -- ?-Oligodeoxynucleotides -- Stereospecific Synthesis of P-Chiral Analogs of Oligonucleotides -- Oligonucleotide Analogs with Dimethylenesulfide, -sulfoxide, and -sulfone Groups Replacing Phosphodiester Linkages -- Oligonucleotide Analogs Containing Dephospho-Internucleoside Linkages -- Scale-Up of Oligonucleotide Synthesis -- Large-Scale Oligonucleotide Synthesis Using the Solid-Phase Approach -- Solid-Phase Supports for Oligonucleotide Synthesis.
520			_aWhen first conceived, not only was the aim of Protocols for Oligo­ nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe­ sizers has become a common practice in numerous laboratories. How­ ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle­ otide analogs have been synthesized and studied for their individual prop­ erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo­ nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.
988			_aSpringer_Protocols_1993
650		7	_2embne _9140525 _aÁcidos nucleicos
776	0	8	_iPrinted edition: _z9781489943927
776	0	8	_iPrinted edition: _z9780896032477
776	0	8	_iPrinted edition: _z9780896032811
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032817 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI