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008			100301s1995 xxu| o |||| 0|eng d
020			_a9781592595426
024	7		_a10.1385/0896033287 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981.5 _b1995 EB
245	0	0	_aPlant Cell Electroporation And Electrofusion Protocols _cedited by Jac A. Nickoloff
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XIV, 206 páginas) _b15 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v55
505	0		_aTheory and Instrumentation -- Electroporation Theory -- Effects of Pulse Length and Strength on Electroporation Efficiency -- Instrumentation -- Electroporation Protocols -- Electroporation of Agrobacterium tumefaciens -- Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii -- Pollen Electrotransformation in Tobacco -- Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA -- Electroporation of Brassica -- Transformation of Maize by Electroporation of Embryos -- Transient Gene Expression Analysis in Electroporated Maize Protoplasts -- Reporter Genes and Transient Assays for Plants -- Electrofusion Protocols -- Electrofusion of Plant Protoplasts -- Protoplast Electrofusion and Regeneration in Potato -- Polymer-Supported Electrofusion of Protoplasts.
520			_aGene transfer is an essential technology for improving our under­ standing of gene structure and function. Although there are many meth­ ods by which DNA may be introduced into cells-including heat and chemical treatments, and microinjection-electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi­ ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in­ cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna­ tives to electric field-based methods, citing the advantages and limita­ tions of the various methods for achieving specific goals.
988			_aSpringer_Protocols_1995
650		7	_2embne _9150279 _aIngeniería genética vegetal
650		7	_2embne _9667619 _aPlantas _xMejora genética
776	0	8	_iPrinted edition: _z9781489940803
776	0	8	_iPrinted edition: _z9781489940797
776	0	8	_iPrinted edition: _z9780896033283
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033287 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI