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007			cr nn 008mamaa
008			150128s2015 xxu| o |||| 0|eng d
020			_a9781493922574
024	7		_a10.1007/978-1-4939-2257-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB147.5 _b2015 EB
245	0	0	_aMitochondrial Medicine : _bVolume I, Probing Mitochondrial Function _cedited by Volkmar Weissig, Marvin Edeas
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XXV, 480 páginas) _b108 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1264
505	0		_aPreparation of 'Functional' Mitochondria: A Challenging Business -- Isolation and Quality Control of Functional Mitochondria -- Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells -- Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels -- Optical Microwell Arrays for Large Scale Studies of Single Mitochondria Metabolic Responses -- Deep Resequencing of Mitochondrial DNA -- Single Cell Analysis of Mitochondrial DNA -- A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome -- Rapid Mitochondrial DNA Isolation Method for Direct Sequencing -- Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR Based Method -- Mitochondria-Targeted RNA Import -- Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR -- mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution -- Simultaneous Quantification of Mitochondrial ATP and ROS Production -- Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine -- Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid -- Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells -- Novel Methods for Measuring the Mitochondrial Membrane Potential -- High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes -- In Vivo Assessment of Mitochondrial Oxygen Consumption -- Imaging Mitochondrial Hydrogen Peroxide in Living Cells -- Simultaneous High-Resolution Measurement of Mitochondrial Respiration and Hydrogen Peroxide Production -- Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells -- Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry -- Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells -- Integrative Methods for Studying Cardiac Energetics -- Computer-Based Prediction of Mitochondria-Targeting Peptides -- Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling -- Assessment of Posttranslational Modification of Mitochondrial Proteins -- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway -- High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase -- Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis -- Analysis of Mitochondrial Dysfunction During Cell Death -- The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions -- Assessment of Mitochondrial Ca2+ Uptake -- Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS -- Characterization of Mitochondrial Populations During Stem Cell Differentiation -- An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons.
520			_aThis expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Methods and Protocols, Volume I: Probing Mitochondrial Function focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Mitochondrial Medicine: Methods and Protocols provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
988			_aSpringer_Protocols_2015
650		7	_2embne _9147980 _aMitocondrias
650		7	_2embne _9414723 _aPatología mitocondrial
776	0	8	_iPrinted edition: _z9781493922567
776	0	8	_iPrinted edition: _z9781493922581
776	0	8	_iPrinted edition: _z9781493955725
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2257-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eIG _zSI