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008			120105s2012 xxu| fo |||| 0|eng d
020			_a9781617794551
024	7		_a10.1007/978-1-61779-455-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050			_aQP551 _b2012 EB
245	0	0	_aTwo Hybrid Technologies _bMethods and Protocols _cedited by Bernhard Suter, Erich E. Wanker.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 329 páginas) _b62 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v812
505	0		_aMatrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems -- Array-Based Yeast Two-Hybrid Screens: A Practical Guide -- High Throughput Yeast Two-Hybrid Screening -- A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein-Protein Interaction Discovery -- High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries -- Virus-Human Cell Interactomes -- Interactome Mapping in Malaria Parasites: Challenges and Opportunities -- Mapping Interactomes with High Coverage and Efficiency Using the Shifted Transversal Design -- Assigning Confidence Scores for Protein-Protein Interactions -- The Intergration and Annotation of the Human Interactome in the UniHI Database -- Gene-Centered Yeast One-Hybrid Assays -- One- plus Two-hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein-Protein Interactions -- Investigation of Membrane Protein Interactions Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System -- Application of the Split-Protein Sensor Trp1 to Protein Interaction Discovery in the Yeast Saccharomyces cerevisiae -- Tetracycline Repressor-Based Mammalian Two-Hybrid Systems -- The Fluorescent Two-Hybrid (F2H) Assay for Direct Analysis of Protein-Protein Interactions in Living Cells -- ArrayMAPPIT: a Screening Platform for Human Protein Interactome Analysis -- MAPPIT as a High-Throughput Screening Assay for Modulators of Protein-Protein Interactions in HIV and HCV -- Integrated Measurement of Split TEV and cis-Regulatory Assays Using EXT Encoded Reporter Libraries.
520			_aProtein-protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols  supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.
988			_aSpringer_Protocols_2012
650		7	_2embne _9140656 _aFisiología animal _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617794544
776	0	8	_iPrinted edition: _z9781617794568
776	0	8	_iPrinted edition: _z9781493958115
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-455-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _ean _zSI