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008			100301s2006 xxu| o |||| 0|eng d
020			_a9781597451130
024	7		_a10.1385/1597451134 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH604.2 _b2006 EB
245	0	0	_aCell'Cell Interactions : _bMethods and Protocols _cedited by Sean P. Colgan
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XIV, 286 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v341
505	0		_aBlood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements -- Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences -- The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells -- Cell-Cell Interactions on Solid Matrices -- Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein ?-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function -- Nucleotide Metabolism and Cell-Cell Interactions -- Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization -- Collagen Gel Contraction Assay -- Methods to Assess Tissue Permeability -- Bacterial-Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy -- A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum -- Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems -- Expression Cloning of Signaling Proteins Regulated by Cell Adhesion -- Assays for the Calcium Sensitivity of Desmosomes -- Tight Junctions and Cell-Cell Interactions -- Simultaneous Immunofluorescence Detection of Cadherin and ?-Catenin in Mouse Tissue Sections -- In Vitro Neutrophil Transepithelial Migration -- The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions -- Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ?-3-Derived Lipid Mediators -- Isolation and Analysis of Lipid Rafts in Cell-Cell Interactions.
520			_aThe use of directed cell models to study the manner in which two or more cells interact has revolutionized our understanding of protein-protein interactions in a complex milieu and has provided an invaluable platform for the identification of new molecules. In Cell-Cell Interactions: Methods and Protocols, highly skilled experts in the development and utilization of cell-cell interaction assays describe in detail their best methods-based approaches, both in vitro and in vivo, for uncovering cellular interactions at the molecular level. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Insightful to beginners and inspiring to seasoned professionals, Cell-Cell Interactions: Methods and Protocols provides the cutting-edge and versatile laboratory tools needed to gain a basic understanding of cell-cell interactions.
988			_aSpringer_Protocols_2006
650		7	_2embne _9667661 _aInteracción celular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617376184
776	0	8	_iPrinted edition: _z9781588295231
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597451134 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI