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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592592944
024	7		_a10.1385/1592592945 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP701 _b2003 EB
245	0	0	_aCapillary Electrophoresis of Carbohydrates _cedited by Pierre Thibault, Susumu Honda
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XIV, 318 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v213
505	0		_aStructural and Functional Diversity of Glycoconjugates -- Sample Preparation Methods -- Chemical and Enzymatic Release of Glycans from Glycoproteins -- Derivatization of Carbohydrates -- Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes -- Analysis of Mono- and Oligosaccharides -- Monosaccharide Analysis by Capillary Electrophoresis -- Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers -- Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis -- Affinity Capillary Electrophoresis of Oligosaccharides -- Characterization of Glycosaminoglycans by Capillary Electrophoresis -- Analysis of Glycoconjugates and the Component Monosaccharides -- Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection -- Separation of Protein Glycoforms by Capillary Electrophoresis -- Separation of Glycoproteins by Capillary Isoelectric Focusing -- Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry -- Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry -- Specific Applications -- Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity -- Determination of Association Constant of Carbohydrate-Protein Interaction.
520			_aRecent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods. Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
988			_aSpringer_Protocols_2003
650		7	_2embne _9143018 _aHidratos de carbono _vManuales de laboratorio
650		7	_2embne _9688491 _aElectroforesis capilar _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617372063
776	0	8	_iPrinted edition: _z9780896038264
776	0	8	_iPrinted edition: _z9781489938473
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592945 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI