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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592593507
024	7		_a10.1385/159259350X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC684 .G44 _b2003 EB
245	0	0	_aCardiac Cell and Gene Transfer _cedited by Joseph M. Metzger
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XI, 253 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v219
505	0		_aVectors for Cardiac Gene Transfer -- Adenoviral Vectors -- Gutted Adenoviral Vectors for Gene Transfer to Muscle -- Dual Vector Expansion of the Recombinant AAV Packaging Capacity -- Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes -- Cell Transfer to Myocardium -- Cell Therapy in the Heart -- Cardiac Cell Transplantation -- Cell Grafting for Cardiac Repair -- Vectors for Cardiac Gene Transfer -- Suppressor tRNAs -- Antisense Oligonucleotides -- Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants -- Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes -- Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes -- In Vivo Cardiac Gene Transfer -- Embryonic and Neonatal Cardiac Gene Transfer In Vivo -- Efficient Viral Gene Transfer to Rodent Hearts In Vivo -- Direct Gene Transfer to the Adult Rodent Myocardium In Vivo -- Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures -- Coronary Perfusion Cocktails for In Vivo Gene Transfer -- Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ?-Adrenergic Receptor Signaling Components -- Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo.
520			_aCellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.
988			_aSpringer_Protocols_2003
650		7	_2embne _9169667 _aCorazón _xEnfermedades _vManuales de laboratorio
650		7	_2embne _9150702 _aTerapia genética _vManuales de laboratorio
700	1		_aMetzger, Joseph M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372926
776	0	8	_iPrinted edition: _z9780896039940
776	0	8	_iPrinted edition: _z9781489938091
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259350X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI