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008			110302s2011 xxu| o |||| 0|eng d
020			_a9781617790430
024	7		_a10.1007/978-1-61779-043-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2011 EB
245	0	0	_aProtein Microarray for Disease Analysis : _bMethods and Protocols _cedited by Catherine J. Wu
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIII, 373 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v723
505	0		_aDetecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays -- Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening -- Antibody Suspension Bead Arrays -- Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies -- Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays -- Recombinant Lectin Microarrays for Glycomic Analysis -- Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection -- SPOT Synthesis as a Tool to Study Protein-Protein Interactions -- Native Antigen Fractionation Protein Microarrays for Biomarker Discovery -- Immunoprofiling Using NAPPA Protein Microarrays -- High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA) -- Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System -- Kinase Substrate Interactions -- A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues -- Multiplexed Detection of Antibodies Using Programmable Bead Arrays -- A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays -- Development of Expression-Ready Constructs for Generation of Proteomic Libraries -- Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection -- Förster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays -- Label-Free Detection with Surface Plasmon Resonance Imaging -- Data Processing and Analysis for Protein Microarrays -- Database Resources for Proteomics-Based Analysis of Cancer.
520			_aProtein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response.  In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease.  These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes.  Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Practical and cutting-edge,  Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617790423
776	0	8	_iPrinted edition: _z9781617790447
776	0	8	_iPrinted edition: _z9781493961740
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-043-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI