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008			150317s2015 xxu| o |||| 0|eng d
020			_a9781493925377
024	7		_a10.1007/978-1-4939-2537-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP620 _b2015 EB
245	0	0	_acAMP Signaling : _bMethods and Protocols _cedited by Manuela Zaccolo
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 222 páginas) _b47 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1294
505	0		_aSimultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters -- Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging -- A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment -- Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters -- Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification -- Channel-Based Reporters for cAMP Detection -- Imaging Sub-Plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters -- Adenoviral Transduction of FRET-Based Biosensors for cAMP in Primary Adult Mouse Cardiomyocytes -- Generation of Transgenic Mice Expressing FRET Biosensors -- Photoactivatable Adenylyl Cyclase (PACs) as a Tool to Study cAMP Signaling In Vivo: An Overview -- Selective Disruption of the AKAP Signaling Complexes -- Screening for Small Molecule Disruptors of AKAP-PKA Interactions -- Structure-Based Bacteriophage Screening for AKAP-Selective PKA Regulatory Subunit Variants -- A Yeast-Based High-Throughput Screen for Modulators of Phosphodiesterase Activity -- Separation of PKA and PKG Signaling Nodes by Chemical Proteomics -- Development of Computational Models of cAMP Signaling.
520			_aThis detailed volume encompasses new technological developments that specifically address questions related to adenosine 3',5'-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system. cAMP, the prototypical intracellular second messenger, regulates a large variety of cellular functions and biological processes, including gene transcription, cell metabolism, proliferation, development, as well as more specialized functions depending on the cell type, so the realization of its extremely complex spatial organization and local regulation is providing novel mechanistic insight into cell physiology and is producing a novel framework for the identification of disease mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, cAMP Signaling: Methods and Protocols serves as a vital resource for researchers working in this expanding, complex field.
988			_aSpringer_Protocols_2015
650		7	_2embne _9140525 _aÁcidos nucleicos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493925384
776	0	8	_iPrinted edition: _z9781493925360
776	0	8	_iPrinted edition: _z9781493949502
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2537-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI