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008			121025s2012 xxu| fo |||| 0|eng d
020			_a9781627031226
024	7		_a10.1007/978-1-62703-122-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK604 _b2012 EB
245	0	0	_aFungal Secondary Metabolism : _bMethods and Protocols _cedited by Nancy P. Keller, Geoffrey Turner
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XII, 288 páginas) _b60 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v944
505	0		_aLibrary Preparation and Data Analysis Packages for Rapid Genome Sequencing -- Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters -- Media and Growth Conditions for Induction of Secondary Metabolite Production -- Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays -- The "FERMEX" Method for Metabolite-Enriched Fungal Extracts -- The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites -- Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri -- Production, Detection, and Purification of Clavine-Type Ergot Alkaloids -- Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) -- Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans -- Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis -- Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host -- Multi-Copy Genetic Screen in Aspergillus nidulans -- Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification -- Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra -- Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi -- Targeted Proteomics for Metabolic Pathway Optimization -- Investigation of In Vivo Protein Interactions in Aspergillus Spores -- Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus -- Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi -- Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects.
520			_aFilamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field.  Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.
988			_aSpringer_Protocols_2012
650		7	_2embne _9138683 _aHongos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627031233
776	0	8	_iPrinted edition: _z9781627031219
776	0	8	_iPrinted edition: _z9781493959600
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-122-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI