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008			100301s2009 xxu| fo |||| 0|eng d
020			_a9781597453943
024	7		_a10.1007/978-1-59745-394-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR201 .H46 _b2009 EB
245	0	0	_aHepatitis C : _bMethods and Protocols _cedited by Hengli Tang
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XXII, 466 páginas) _b74 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v510
505	0		_aDetection and Quantification of HCV -- Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays -- Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen -- Immunohistochemical Detection of HCV Proteins in Liver Tissue -- HCV Nomenclature, Genotyping, and Quasispecies -- Nomenclature and Numbering of the Hepatitis C Virus -- A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates -- Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry -- HCV Replication in Vitro -- Purification and Crystallization of NS5A Domain I of Hepatitis C Virus -- NS5A Phosphorylation and Hyperphosphorylation -- Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies -- Structural and Functional Analysis of the HCV p7 Protein -- HCV Replicons: Overview and Basic Protocols -- Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA -- Studying HCV RNA Synthesis In Vitro with Replication Complexes -- Proteomics Study of the Hepatitis C Virus Replication Complex -- Investigation of the Hepatitis C Virus Replication Complex -- Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease -- Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay -- High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System -- Inhibition of HCV Replication by Small Interfering RNA -- HCV Entry and Infection -- Cellular Receptors and HCV Entry -- Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp) -- Screening of Small-Molecule Compounds as Inhibitors of HCV Entry -- Isolation of JFH-1 Strain and Development of an HCV Infection System -- Measuring HCV Infectivity Produced in Cell Culture and In Vivo -- Genotype 1a HCV (H77S) Infection System -- Full-Length Infectious HCV Chimeras -- Adaptation of the Hepatitis C Virus to Cell Culture -- Primary Human Hepatocyte Culture for HCV Study -- In Vivo Study of HCV in Mice with Chimeric Human Livers -- Immunity and Modeling -- Determination of HCV-Specific T-Cell Activity -- Analysis of HCV-Specific T Cells by Flow Cytometry -- Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp) -- Mathematical Modeling of HCV Infection and Treatment.
520			_aWith the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.
988			_aSpringer_Protocols_2009
650		7	_2embne _9145703 _aHepatitis víricas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607610892
776	0	8	_iPrinted edition: _z9781617378591
776	0	8	_iPrinted edition: _z9781588299703
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-394-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b09/2023 _dz _eu _zSI