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008			110406s2011 xxu| fo |||| 0|eng d
020			_a9781617791086
024	7		_a10.1007/978-1-61779-108-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH631 _b2011 EB
245	0	0	_aMammalian Cell Viability : _bMethods and Protocols _cedited by Martin J. Stoddart
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 240 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v740
505	0		_aCell Viability Assays: Introduction -- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods -- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay -- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells -- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye -- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability -- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays -- Annexin V / 7-AAD Staining in Keratinocytes -- Measurement of Caspase Activity: From Cell Populations to Individual Cells -- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes -- Multiplexing Cell Viability Assays -- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death -- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs -- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers -- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections -- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay -- Exploiting the Liberation of Zn2+ to Measure Cell Viability -- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs -- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test -- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses -- Digital Image Processing of Live/Dead Staining.
520			_aWhether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
988			_aSpringer_Protocols_2011
650		7	_2embne _9165668 _aFisiología celular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617791079
776	0	8	_iPrinted edition: _z9781617791093
776	0	8	_iPrinted edition: _z9781493961535
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-108-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI