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007			cr nn 008mamaa
008			100301s2007 xxu| fo |||| 0|eng d
020			_a9781597452946
024	7		_a10.1007/978-1-59745-294-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH211 _b2007 EB
245	0	0	_aElectron Microscopy : _bMethods and Protocols _cedited by John Kuo
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XVI, 608 páginas) _b214 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v369
505	0		_aConventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells -- Processing Biological Tissues for Ultrastructural Study -- Processing Plant Tissues for Ultrastructural Study -- Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy -- Ultramicrotomy for Biological Electron Microscopy -- Poststaining Grids for Transmission Electron Microscopy -- Negative Staining of Thinly Spread Biological Samples -- Recent Advances in High-Pressure Freezing -- Cryoultramicrotomy -- Cell-Free Extract Systems and the Cytoskeleton -- Electron Microscopy In Situ Hybridization -- Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections -- Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry -- Fluorescence-Integrated Transmission Electron Microscopy Images -- Quantitative Immunoelectron Microscopy -- Electron Crystallography of Membrane Proteins -- Cryoelectron Microscopy of Icosahedral Virus Particles -- Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography -- Whole-Mount Immunoelectron Tomography of Chromosomes and Cells -- Three-Dimensional Cryotransmission Electron Microscopy of Cells and Organelles -- Electron Energy-Loss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens -- Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens -- Variable Pressure and Environmental Scanning Electron Microscopy -- Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy -- X-Ray Microanalysis in the Scanning Electron Microscope -- Botanical X-Ray Microanalysis in Cryoscanning Electron Microscopy -- Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research -- Using SIMS and MIMS in Biological Materials.
520			_aIn this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.
988			_aSpringer_Protocols_2007
650		7	_2embne _9677489 _aMicroscopios _vManuales de laboratorio
650		7	_2embne _9671675 _aElectrones _vManuales de laboratorio
650		7	_2embne _9138721 _aMicroscopia electrónica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627038140
776	0	8	_iPrinted edition: _z9781588295736
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-294-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI