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007			cr nn 008mamaa
008			110414s2009 xxu| fo |||| 0|eng d
020			_a9781597455589
024	7		_a10.1007/978-1-59745-558-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2009 EB
245	0	0	_aRiboswitches : _bMethods and Protocols _cedited by Alexander Serganov
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XI, 366 páginas) _b65 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v540
505	0		_aPredicting Riboswitch Regulation on a Genomic Scale -- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications -- Application of Fluorescent Measurements for Characterization of Riboswitch-Ligand Interactions -- Transcriptional Approaches to Riboswitch Studies -- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription -- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches -- Methods for Analysis of Ligand-Induced RNA Conformational Changes -- Monitoring RNA-Ligand Interactions Using Isothermal Titration Calorimetry -- Preparation and Crystallization of Riboswitch-Ligand Complexes -- Crystallization of the glmS Ribozyme-Riboswitch -- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering -- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches -- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension -- Identification of Metabolite-Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression -- RNA-Dependent RNA Switches in Bacteria -- Probing mRNA Structure and sRNA-mRNA Interactions in Bacteria Using Enzymes and Lead(II) -- Structural Probing of RNA Thermosensors -- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria -- Isolation and Characterization of the Heat Shock RNA 1 -- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo -- In Vitro Selection of Conformational Probes for Riboswitches -- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo -- High-Throughput Screens to Discover Synthetic Riboswitches -- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage -- In Vitro Selection of glmS Ribozymes.
520			_aThe revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology.
988			_aSpringer_Protocols_2009
650		7	_2embne _9144940 _aARN _vManuales de laboratorio
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781603278812
776	0	8	_iPrinted edition: _z9781617379475
776	0	8	_iPrinted edition: _z9781934115886
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-558-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eu _zSI