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020 _a9781493978939
024 7 _a10.1007/978-1-4939-7893-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP551
_b2018 EB
245 0 0 _aProtein Scaffolds :
_bDesign, Synthesis, and Applications
_cedited by Andrew K. Udit
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 330 páginas)
_b76 ilustraciones, 52 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1798
505 0 _aNanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus -- Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles -- Encapsulation of Exogenous Proteins in Vault Nanoparticles -- Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage -- Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules -- Modification of CCMV Nanocages for Enzyme Encapsulation -- Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization -- Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates -- TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings -- TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings -- Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins -- Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis -- Incorporation of Non-canonical Amino Acids into Proteins by Global Reassignment of Sense Codons -- Modification of Protein Scaffolds via Copper-catalyzed Azide-Alkyne Cycloaddition -- Antibody Modification of p-aminophenylalanine-containing Proteins -- Cross-linked Collagen Gels using Gold Nanoparticles -- Genipin Cross-linking Of Elastin and Elastin-based Proteins -- Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides -- Design and Characterization of Fibers and Bionanocomposites Using the Coiled-coil Domain of Cartilage Oligomeric Matrix Protein -- Preparing Genes for Repetitive Elastin-like Polypeptides Using Gibson Assembly -- Peptide Amphiphile Micelles for Vaccine Delivery -- Controlled Assembly of the Filamentous Chaperone Gamma-prefoldin into Defined Nanostructures -- Advances in the Application of Designed Ankyrin Repeat Proteins (Darpins) As Research Tools and Protein Therapeutics. .
520 _aThis volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field.
988 _aSpringer_Protocols_2018
650 7 _2embne
_9139861
_aProteínas
650 7 _2embne
_9140931
_aBiotecnología
776 0 8 _iPrinted edition:
_z9781493978922
776 0 8 _iPrinted edition:
_z9781493978946
776 0 8 _iPrinted edition:
_z9781493993178
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7893-9
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eb
_zSI