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008			111104s2012 xxua o |||| 0|eng d
020			_a9781617793738
024	7		_a10.1007/978-1-61779-373-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP701 _b2021 EB
245	0	0	_aCarbohydrate Microarrays : _bMethods and Protocols _cedited by Yann Chevolot
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XV, 427 páginas) _b137 ilustraciones, 28 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v808
505	0		_aRecent Advances and Future Challenges in Glycan Microarray Technology -- Chemical Synthesis of Carbohydrates and Their Surface Immobilisation: A Brief Introduction -- General Consideration on Sialic Acid Chemistry -- Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates -- Polypyrrole-Oligosaccharide Microarray for the Measurement of Biomolecular Interactions by Surface Plasmon Resonance Imaging -- Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis -- Carbohydrate Microarrays for Enzymatic Reactions and Quantification of Binding Affinities for Glycan-Protein Interactions -- Neoglycolipid (NGL)-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Non-Covalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses -- Preparation of a Mannose-6-phosphate Glycan Microarray Through Fluorescent Derivatization, Phosphorylation, and Immobilization of Natural High-mannose N-glycans and its Application in Ligand Identification of P-type Lectins -- Production of Fluorous-Based Microarrays with Uncharged Carbohydrates -- General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides -- Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays -- Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray -- Glycoarray by DNA Directed Immobilization -- Fabrication of Carbohydrate Surfaces by Using Non Derivatised Oligosaccharides --  Polysaccharide Microarrays. Application to the Identification of Heparan Sulfate Mimetics -- Carbohydrate Antigen Microarrays -- Probing Virus-Glycan Interactions Using Glycan Microarrays -- MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays -- Microarray Technology using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection -- Studying Modification of Aminoglycoside Antibiotics by Resistance-causing Enzymes via Microarray -- Microarray Method for the Rapid Detection of Glycosaminoglycan-Protein Interactions -- Neoglycolipid-based 'Designer' Oligosaccharide Microarrays to Define b-glucan Ligands for Dectin-1 -- Measurement of Antibodies to Pneumococcal Polysaccharides with Luminex xMAP Microsphere-based Liquid Arrays -- Carbohydrate Microarrays in 96-well Polystyrene Microtiter Plates -- Photoimmobilization of Saccharides -- Microwave Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydrazide-Dendrimer Substrate -- Combinatorial Glycoarray.
520			_aCarbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe. .
988			_aSpringer_Protocols_2012
650		7	_2embne _9143018 _aHidratos de carbono _vManuales de carbono
776	0	8	_iPrinted edition: _z9781617793721
776	0	8	_iPrinted edition: _z9781617793745
776	0	8	_iPrinted edition: _z9781493958665
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-373-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI