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008			100301s2001 xxua o |||| 0|eng d
020			_a9781592592081
024	7		_a10.1385/1592592082 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP624.75 .P74 _b2001 EB
245	0	0	_aDNA'Protein Interactions : _bPrinciples and Protocols _cedited by Tom Moss
250			_a2nd edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (656 páginas) _b146 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v148
505	0		_aFilter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Uranyl Photofootprinting -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ultraviolet-Laser Footprinting -- In Vivo DNA Analysis -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Ethylation Interference -- Hydroxyl Radical Interference -- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Chemical Modification of Lysine by Reductive Methylation -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Site-Specific Protein-DNA Photocrosslinking -- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex -- UV Laser-Induced Protein-DNA Crosslinking -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Engineering Nucleic Acid-Binding Proteins by Phage Display -- Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast -- Assays for Transcription Factor Activity -- Assay of Restriction Endonucleases Using Oligonucleotides -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Calorimetry of Protein-DNA Complexes and Their Components -- Surface Plasmon Resonance Applied to DNA-Protein Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Two-Dimensional Crystallization of Soluble Protein Complexes -- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Scanning Transmission Electron Microscopy of DNA-Protein Complexes -- Determination of Nucleic Acid Recognition Sequences by SELEX -- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation.
520			_aDNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.
988			_aSpringer_Protocols_2001
650		7	_2embne _9667661 _aInteracción celular _vManuales de laboratorio
650		7	_2embne _9140806 _aADN _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9780896036253
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592082 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI