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007			cr nn 008mamaa
008			100301s2009 xxu| o |||| 0|eng d
020			_a9781597454933
024	7		_a10.1007/978-1-59745-493-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2009 EB
245	0	0	_aMass Spectrometry of Proteins and Peptides : _bMethods and Protocols _cedited by Mary S. Lipton, Ljiljana Paša-Tolic
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XIV, 470 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v492
505	0		_aGeneration and Analysis of Multidimensional Protein Identification Technology Datasets -- Quantitative Peptide and Protein Profiling by Mass Spectrometry -- Label-Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach -- Classical Proteomics: Two-Dimensional Electrophoresis/MALDI Mass Spectrometry -- The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression -- Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins -- High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation -- Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra -- Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry -- Structure Analysis of N -Glycoproteins -- Capillary Zone Electrophoresis-Mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins -- Top-Down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer -- Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry -- Integrating Accelerated Tryptic Digestion into Proteomics Workflows -- Hydrogen/Deuterium Exchange Mass Spectrometry -- Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes -- Chemical Cross-Linking for Protein-Protein Interaction Studies -- Tissue Analysis with High-Resolution Imaging Mass Spectrometry -- Proteomic Global Profiling for Cancer Biomarker Discovery -- Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation, Protein Microarray Technology, and Mass Spectrometry -- Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid -- Characterization of Microorganisms by MALDI Mass Spectrometry -- Mass Spectrometric Characterization of Neuropeptides -- Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods -- Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics -- Two-Dimensional Ion Mobility Analyses of Proteins and Peptides -- Proteomics for Validation of Automated Gene Model Predictions -- Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search.
520			_aWhen the last edition of this book was published in 2000, the field of proteomics was in its infancy. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. As a result of these rapid developments that have led to expanded areas of research and innovative applications, an update to Mass Spectrometry of Proteins and Peptides is not only worthwhile, but arguably mandatory. Spanning fields from microbial forensics and clinical applications to protein structure, dynamics and function, the following chapters written by leading experts serve as a forum for presenting some of the latest revolutionizing methods for mass spectrometry-based characterization of proteins and peptides. Staying faithful to the spirit of the Methods in Molecular Biology™ series, all chapters are written in the same format, whereby each chapter opens with a basic description of the method, followed by a description of the protocol and then practical step-by-step instructions to ensure experimental success. Mass Spectrometry of Proteins and Peptides: Methods and Protocols, Second Edition is a valuable resource for novice and seasoned practitioners in the fields of biochemistry, molecular biology, clinical chemistry, immunology, genetic, microbiology and toxicology.
988			_aSpringer_Protocols_2009
650		7	_2embne _9242917 _aProteínas _xAnálisis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607611042
776	0	8	_iPrinted edition: _z9781934115480
776	0	8	_iPrinted edition: _z9781627037969
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-493-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI