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007			cr nn 008mamaa
008			110907s2011 xxua o |||| 0|eng d
020			_a9781617792892
024	7		_a10.1007/978-1-61779-289-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450 _b2011 EB
245	0	0	_aGene Expression Profiling : _bMethods and Protocols _cedited by Lorraine O'Driscoll
250			_a2nd edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 200 páginas) _b37 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v784
505	0		_aReal-Time PCR and Multiplex Approaches -- Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs -- Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals -- Software and Tools for Microarray Data Analysis -- Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells -- Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay -- MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs -- Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases -- 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins -- Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis -- Immunohistochemical and Immunofluorescence Procedures for Protein Analysis -- Advanced Microscopy: Laser Scanning Confocal Microscopy -- Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling -- Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties.
520			_aUnderstanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.
988			_aSpringer_Protocols_2011
650		7	_2embne _9162770 _aExpresión génica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617792885
776	0	8	_iPrinted edition: _z9781617792908
776	0	8	_iPrinted edition: _z9781493961733
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-289-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI