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007			cr nn 008mamaa
008			100316s2010 xxua o |||| 0|eng d
020			_a9781597450195
024	7		_a10.1007/978-1-59745-019-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.27 _b2010 EB
245	0	0	_aMouse Cell Culture : _bMethods and Protocols _cedited by Andrew Ward, David Tosh
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XI, 250 páginas) _b48 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v633
505	0		_aThe Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies -- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures -- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells -- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference -- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation -- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine -- Isolation and Culture of Embryonic Pancreas and Liver -- Isolation and Culture of Mouse Satellite Cells -- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures -- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes -- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells -- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes -- Isolation and Culture of Adult Mouse Hepatocytes -- Isolation and Culture of Mouse Intestinal Cells -- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse -- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry -- Cell Culture of Primary Cerebellar Granule Cells -- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain.
520			_aCultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
988			_aSpringer_Protocols_2010
650		7	_2embne _9140931 _aBiotecnología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607616955
776	0	8	_iPrinted edition: _z9781588297723
776	0	8	_iPrinted edition: _z9781493960866
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-019-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI