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008			100301s1995 xxua o |||| 0|eng d
020			_a9781592595341
024	7		_a10.1385/0896033104 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.5 .E48 _b1995 EB
245	0	0	_aElectroporation Protocols for Microorganisms _cedited by Jac A. Nickoloff
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XIX, 372 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v47
505	0		_aElectroporation Theory -- Instrumentation -- Direct Plasmid Transfer Between Bacterial Species and Electrocuring -- Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation -- Production of cDNA Libraries by Electroporation -- Electroporation of RNA into Saccharomyces cerevisiae -- Electrofusion of Yeast Protoplasts -- Escherichia coli Electrotransformation -- Electrotransformation in Salmonella -- Electrotransformation of Pseudomonas -- Electroporation of Xanthomonas -- Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids -- Electroporation of Francisella tularensis -- A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation -- Genetic Transformation of Bacteroides spp. Using Electroporation -- Electrotransformation of Agrobacterium -- Electroporation of Helicobacter pylori -- Electrotransformation of Streptococci -- Transformation of Lactococcus by Electroporation -- Transformation of Lactobacillus by Electroporation -- Electrotransformation of Staphylococci -- Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine -- of Recombinant DNA into Clostridium spp. -- Electroporation of Mycobacteria -- Electrotransformation of the Spirochete Borrelia burgdorferi -- Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation -- Ten-Minute Electrotransformation of Saccharomyces cerevisiae -- Electroporation of Schizosaccharomyces pombe -- Gene Transfer by Electroporation of Filamentous Fungi -- Transformation of Candida maltosa by Electroporation -- Electroporation of Physarum polycephalum -- Electroporation of Dictyostelium discoideum -- Gene Transfer by Electroporation in Tetrahymena -- Transfection of the African and American Trypanosomes -- Electroporation in Giardia lamblia.
520			_aElectroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.
988			_aSpringer_Protocols_1995
650		7	_2embne _9166658 _aElectroporación _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781489940766
776	0	8	_iPrinted edition: _z9780896033108
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033104 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zPRE