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008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592596775
024	7		_a10.1385/1592596770 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH452.8 _b2000 EB
245	0	0	_aIn Situ Hybridization Protocols _cedited by Ian A. Darby
250			_a2nd edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XIX, 480 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v123
505	0		_aChromosomal Techniques -- Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids -- DNA-Protein In Situ Covisualization for Chromosome Analysis -- Radioactive In Situ Hybridization to Animal Chromosomes -- Native Polytene Chromosomes of Drosophila melanogaster for Light and Electron Microscopic Observation of the Conformation and Distribution of Molecules -- Released Chromatin or DNA Fiber Preparations for High-Resolution Fiber FISH -- In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species -- In Situ Hybridization to Polytene Chromosomes -- Comparative Gene Mapping in Exotic Species Using FISH -- Tissue Techniques -- In Situ Hybridization with Nonradioactive Probes -- Quantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Heterocellular Systems -- In Situ End-Labeling of Fragmented DNA and the Localization of Apoptosis -- Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections -- in situ Hybridization Protocols for Detection of Viral DNA Using Radioactive and Nonradioactive DNA Probes -- Signal Amplification for DNA and mRNA -- In Situ Localization of PCR-Amplified DNA and cDNA -- Ultrastructural Detection of Nucleic Acids on Thin Sections of Tissue Embedded in Hydrophilic Resin -- In Situ Hybridization for Electron Microscopy -- In Situ Hybridization of Whole-Mount Embryos -- cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods -- Applications -- Molecular Cytogenetic Analysis of Sperm from Infertile Males Undergoing Intracytoplasmic Sperm Injection -- Localization of HIV-1 DNA and Tumor Necrosis Factor-? mRNA in Human Brain Using Polymerase Chain Reaction In Situ Hybridization and Immunocytochemistry.
520			_aInIn Situ Hybridization Protocols, 2nd Edition, Ian Darby updates the highly successful 1st edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Each protocol provides detailed, easy-to-follow instructions, along with realistic commentaries on how to vary their application and how to troubleshoot problems that arise. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire .
988			_aSpringer_Protocols_2000
650		7	_2embne _9688531 _aHibridación in situ _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781489928047
776	0	8	_iPrinted edition: _z9781489928030
776	0	8	_iPrinted edition: _z9780896036864
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596770 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI