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008			110201s2011 xxua o |||| 0|eng d
020			_a9781617379796
024	7		_a10.1007/978-1-61737-979-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .T2 _b2011 EB
245	0	0	_aRegulatory T Cells : _bMethods and Protocols _cedited by George Kassiotis, Adrian Liston
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 283 páginas) _b44 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v707
505	0		_aRegulatory T Cells: History and Perspective -- In vitro Treg Suppression Assays -- Generation of T Cell Hybridomas from Naturally-Occurring FoxP3+ Regulatory T Cells -- In vitro and In vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells -- Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus -- ChIP-on-Chip for FoxP3 -- Live Imaging of Dendritic Cell-Treg Cell Interactions -- Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In vivo -- In vivo Treg Suppression Assays -- In vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model -- Antigen-Specific Induction of Regulatory T Cells In vivo and In vitro -- In vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection -- Analysis of Human FoxP3+ Treg Cells Phenotype and Function -- Depletion of Human Regulatory T Cells -- Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In vitro Assay -- Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose -- Flow Cytometric Detection of Human Regulatory T Cells.
520			_aDriven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.
988			_aSpringer_Protocols_2011
650		7	_2embne _9144344 _aLinfocitos T _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617379789
776	0	8	_iPrinted edition: _z9781617379802
776	0	8	_iPrinted edition: _z9781493961580
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-979-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI