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020 _a9781627034623
024 7 _a10.1007/978-1-62703-462-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.25 .N35
_b2013 EB
245 0 0 _aNanomaterial Interfaces in Biology :
_bMethods and Protocols
_cedited by Paolo Bergese, Kimberly Hamad-Schifferli
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 293 páginas)
_b73 ilustraciones, 53 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1025
505 0 _aPreparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications -- DNA Conjugation to Nanoparticles -- Conjugation of Nanoparticles to Proteins -- Water-Solubilization and Functionalization of Semiconductor Quantum Dots -- Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates -- Reliable Methods for Silica Coating of Au Nanoparticles -- Surface Modifications by Polymers for Biomolecule Conjugation -- Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors -- Stability and Aggregation Assays of Nanoparticles in Biological Media -- Electrochemical Measurements of DNA Melting on Surfaces -- Formation and Characterization of Nanoparticle-Protein Corona -- Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution -- Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment -- Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles -- Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption -- Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles -- Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging -- Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy -- Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs -- Modification of Carbon Nanotubes for Gene Delivery Vectors -- Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors -- Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation.
520 _aThe intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9158453
_aNanotecnología
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781627034630
776 0 8 _iPrinted edition:
_z9781627034616
776 0 8 _iPrinted edition:
_z9781493960071
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-462-3
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eb
_zSI