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008			100301s1993 xxu| o |||| 0|eng d
020			_a9781592596300
024	7		_a10.1385/0896032493 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .P4 _b1993 EB
245	0	0	_aNeurotrophic Factors _cedited by Alan A. Boulton, Glen B. Baker, Franz Hefti
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (XVIII, 422 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v25
505	0		_aPurification and Microsequencing of Neurotrophic Proteins -- Identification of Neurotrophic Factors by Molecular Biological Techniques -- Visualization and Quantitation of Neurotrophin mRNAs -- Molecular Analysis of Neurotrophin Genes -- Production of Recombinant Neurotrophic Factors -- Production of Genetically Engineered Cells Releasing Neurotrophic Factors -- Characterization and Localization of Receptors for Neurotrophic Factors -- Transgenic Expression of Neurotrophic Factors and Their Receptors -- Retrograde Axonal Transport -- Experimental Systems to Study Neurotrophic Factor Effects on Rat Brain Cells -- Nonhuman Primate Models in Trophic Factor Research -- Models to Evaluate Effects of Neurotrophic Factors on Axonal Regeneration.
520			_aThe field of neurotrophic factors has witnessed exp- sive growth in the past decade. As is usual in scientific in- vation, this progress has been closely associated with methodological advances. The introduction of molecular b- logical techniques into the neurotrophic factor field led to the discovery of new families of neurotrophic growth f- tors and their receptors. Production of growth factors by recombinant technology played a crucial part. The example of nerve growth factor, the paradigmatic neurotrophic factor, illustrates this point. A decade ago investigators were forced to purify small quantities of this protein from murine salivary glands, but much larger qu- tities of recombinant nerve growth factor are now available for experimentation as well as clinical development. A decade ago there was a controversy about the existence of nerve growth factor in the brain and the immunoassays used for its measurement, but current publications report the precise localization of gene expression for nerve growth factor and its receptor in the brain. Neurotrophic Factors aims at presenting the techniques that have been crucial to the realization of these rapid advances and thus have helped propel the neurotrophic factors field to its current status of high visibility. These techniques range from molecular biological methods used for cloning and production, to cell culture methods for assessing biological activities, to animal models of nervous system injury (nec- sary for the development of therapeutic agents from neurotrophic factors).
988			_aSpringer_Protocols_1993
650		7	_2embne _9146243 _aPéptidos
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781489939876
776	0	8	_iPrinted edition: _z9780896032491
776	0	8	_iPrinted edition: _z9781489939869
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032493 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI