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020			_a9781592598113
024	7		_a10.1385/1592598110 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC268.5 _b2004 EB
245	0	0	_aCheckpoint Controls and Cancer _nVolume 2, _pActivation and Regulation Protocols _cedited by Axel H. Schönthal.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XVIII, 358 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v281
505	0		_aProtocols for the Study of Checkpoint Regulatory Components -- Analysis of RB Action in DNA Damage Checkpoint Response -- Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle -- Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis -- Functional Analysis of CDK Inhibitor p21WAF1 -- Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response -- Quantitative Determination of p16 Gene Expression by RT-PCR -- Measuring Cyclin-Dependent Kinase Activity -- Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase-Related Kinase Family -- CHK1 Kinase Activity Assay -- Assaying Cdc25 Phosphatase Activity -- Analyzing the Regulation and Function of ATM -- Use of siRNA to Study the Function of MDC1 in DNA Damage Responses -- Functional Analysis of APC-Cdh1 -- Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells -- Analysis of the Spindle-Assembly Checkpoint in HeLa Cells -- Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay -- Studying Consequences of Checkpoint Pathway Activation -- Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors -- Biochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic Beads -- Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates -- Analyzing Cell Cycle Checkpoints After Ionizing Radiation -- FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells -- Analysis of Cell Cycle by Flow Cytometry -- Analyzing Markers of Apoptosis In Vitro -- Analysis of Telomerase Activity and Telomere Function in Cancer.
520			_aIntracellular checkpoint controls constitute a network of signal transduction pathways that protect cells from external stresses and internal errors by means of cell cycle arrest, DNA repair, or apoptosis. Failure of this machinery can lead to embryonic death, genetic diseases, and cancer. In Checkpoint Controls and Cancer, Volume 2: Activation and Regulation Protocols, Axel H. Schönthal, PhD, marshals a prestigious panel of researchers working at the cutting edges of their fields to present readily reproducible experimental protocols for the study of the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these powerful techniques offer such novel approaches as the use of genome databases and siRNA to analyze how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. The earlier first volume, Reviews and Model Systems, comprehensively describes the complexities of checkpoint controls and the model systems available to study them. Comprehensive and up-to-date, the two volumes of Checkpoint Controls and Cancer offer novice and experienced researchers alike not only entré into the complexities of this vast field, but also to the full panoply of productive tools needed to deepen understanding of the systems, as well as to develop new and more effective therapies.
988			_aSpringer_Protocols_2004
650		7	_2embne _9146492 _aCarcinogénesis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617376030
776	0	8	_iPrinted edition: _z9781588295002
776	0	8	_iPrinted edition: _z9781489943668
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598110 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI