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008			170331s2017 xxua o |||| 0|eng d
020			_a9781493968176
024	7		_a10.1007/978-1-4939-6817-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQD381 _b2017 EB
245	0	0	_aMorpholino Oligomers : _bMethods and Protocols _cedited by Hong M. Moulton, Jon D. Moulton
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 284 páginas) _b60 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1565
505	0		_aInvention and Early History of Morpholinos: From Pipe Dream to Practical Products -- Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose -- Aggregation and Disaggregation of Morpholino Oligomers in Solution -- End-Modifications on Morpholino Oligos -- Inducible Inhibition of Gene Function with Photomorpholinos -- Blocking Zebrafish microRNAs with Morpholinos -- Using Morpholinos to Examine Gene Function During Fin Regeneration -- Using Morpholinos to Probe Gene Networks in Sea Urchin -- Manipulating Gene Expression in the Chick Embryo -- Inhibition of Bacterial Growth by Peptide-conjugated Morpholino Oligomers -- Use of Translation Blocking Morpholinos for Gene Knock-Down in Giardia lamblia -- Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos -- Targeting Functional Non-coding RNAs -- Use of Morpholino Oligomers for Pretargeting -- Diagnostic Applications of Morpholinos and Label-free Electrochemical Detection of Nucleic Acids -- Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice -- Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy -- In vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.<intracerebroventricular delivery="" in="" mice="" for="" motor="" neuron="" diseases.-="" of="" morpholino="" antisense="" oligonucleotides="" to="" a="" developing="" ovine="" conceptus="" via="" luminal="" injection="" into="" ligated="" uterine="" horn.-="" surface="" plasmon="" resonance-based="" concentration="" determination="" assay:="" label-free="" and="" antibody-free="" quantification="" morpholinos.-="" ultrasensitive="" hybridization-based="" elisa="" method="" the="" phosphorodiamidate="" biological="" samples. .
520			_aThis volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.
988			_aSpringer_Protocols_2017
650		7	_2embne _9145067 _aMacromoléculas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493968152
776	0	8	_iPrinted edition: _z9781493968169
776	0	8	_iPrinted edition: _z9781493983070
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6817-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI