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007			cr nn 008mamaa
008			100301s2008 xxu| o |||| 0|eng d
020			_a9781597451826
024	7		_a10.1007/978-1-59745-182-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP92 _b2008 EB
245	0	0	_aHematopoietic Stem Cell Protocols _cedited by Kevin D. Bunting
250			_a2nd edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIV, 312 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v430
505	0		_aOverview -- The Expanding Tool Kit for Hematopoietic Stem Cell Research -- Stem Cell Enrichment and Analysis -- Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties -- Mobilization of Hematopoietic Stem and Progenitor Cells in Mice -- Transmigration of Human CD34+ Cells -- Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells -- Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry -- In Vitro Assays and Differentiation -- In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells -- Hematopoietic Development of Human Embryonic Stem Cells in Culture -- In Vitro Human T Cell Development Directed by Notch-Ligand Interactions -- In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C -- Transplantation Assays for Mouse and Human HSC -- Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection -- Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects -- Isolation and Functional Characterization of Side Population Stem Cells -- Transplantation of Chimeric Fetal Liver to Study Hematopoiesis -- Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair -- Genetic Modification of HSCs and Imaging Engraftment -- Retroviral Transduction of Murine Hematopoietic Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Stem Cells -- Retroviral Integration Site Analysis in Hematopoietic Stem Cells -- A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells -- Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.
520			_aWith new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology™ series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation.
988			_aSpringer_Protocols_2008
650		7	_2embne _9165039 _aCélulas madre hematopoyéticas
776	0	8	_iPrinted edition: _z9781617378164
776	0	8	_iPrinted edition: _z9781588298683
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-182-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI