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008			100301s1998 xxu o |||| 0|eng d
020			_a9781592595822
024	7		_a10.1385/1592595820 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .L5 _b1998 EB
245	0	0	_aLipoprotein Protocols _cedited by Jose M. Ordovas
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XI, 284 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v110
505	0		_aDNA- and RNA-Based Methodolog -- PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis -- Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis -- Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method -- Generation of Transgenic Mice -- Preparation of Knockout Mice -- Protein-Based Methodology -- Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins -- Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC) -- Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis -- Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity -- Electrophoretic Separation of LDL and HDL Subclasses -- Measurement of Lipoprotein Particles -- Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins -- Separation and Quantitation of Phospholipid Classes by HPLC -- Assays of Lecithin Cholesterol Acyltransferase (LCAT) -- Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP) -- In Vitro Measurement of Lipoprotein and Hepatic Lipases -- Cellular Assays for Lipoprotein Receptors -- Measurements of Proteoglycan-Lipoprotein Interaction by Gel Mobility Shift Assay.
520			_aIn Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.
988			_aSpringer_Protocols_1998
650		7	_2embne _9146390 _aLipoproteínas _xInvestigacón
776	0	8	_iPrinted edition: _z9781617370311
776	0	8	_iPrinted edition: _z9780896034204
776	0	8	_iPrinted edition: _z9781489942708
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592595820 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI