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008			100301s1999 xxu| o |||| 0|eng d
020			_a9781592595815
024	7		_a10.1385/1592595812 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP608 _b1999 EB
245	0	0	_aLipase and Phospholipase Protocols _cedited by Mark Doolittle
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XVII, 362 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v109
505	0		_aLipase Activity, Purification, and Expression -- Phospholipase A2 and Phosphatidylinositol-Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate -- Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates -- Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate -- Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2 -- Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures -- Human Plasma Platelet-Activating Factor Acetylhydrolase -- Assays for Pancreatic Triglyceride Lipase and Colipase -- Bile Salt-Activated Lipase -- Determining Lipoprotein Lipase and Hepatic Lipase Activity Using Radiolabeled Substrates -- Lysosomal Acid Lipase -- Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase -- Lecithin-Cholesterol Acyltransferase -- Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatography -- Large-Scale Lipoprotein Lipase Purification from Adipose Tissue -- Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B -- High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells -- High-Level Baculoviral Expression of Hormone-Sensitive Lipase -- High-Level Baculoviral Expression of Lysosomal Acid Lipase -- One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells -- Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides -- Biochemical Characterization -- Immunodetection of Lipoprotein Lipase: Antibody Production, Immunoprecipitation, and Western Blotting Techniques -- Immunological Characterization of Digestive Lipases -- Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation -- Detecting Ligands Interacting with Lipoprotein Lipase -- Monolayer Techniques for Studying Lipase Kinetics -- Efficient Immobilization of Phospholipase A2 -- Molecular Genetic Characterization -- An Introduction to Internet Resources for the Molecular and Genetic Analysis of the Lipases -- Techniques for the Measurement of Lipoprotein Lipase Messenger RNA -- In Vitro Transcription and Translation of Lipoprotein Lipase -- Induced Lipase Mutations in the Mouse.
520			_aLipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes.
988			_aSpringer_Protocols_1999
650		7	_2embne-DP _9146752 _aHidrolasas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617370694
776	0	8	_iPrinted edition: _z9780896035461
776	0	8	_iPrinted edition: _z9781489943286
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592595812 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI