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008			100301s2006 xxua o |||| 0|eng d
020			_a9781597451444
024	7		_a10.1385/1597451444 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK603 _b2006 EB
245	0	0	_aDictyostelium discoideum Protocols _cedited by Ludwig Eichinger
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XVI, 564 páginas) _b186 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v346
505	0		_aThe Organism, Community Resources, and Genome-wide Studies -- The Secret Lives of Dictyostelium -- The Genome of Dictyostelium discoideum -- The cDNA Sequencing Project -- dictyBase and the Dicty Stock Center -- Analysis of Gene Expression Using cDNA Microarrays -- Proteomic Analysis of Dictyostelium discoideum -- Basic Methods and Molecular Genetic Techniques -- Cultivation, Spore Production, and Mating -- Parasexual Genetics Using Axenic Cells -- Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior -- Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes -- Generation of Multiple Knockout Mutants Using the Cre-loxP System -- Restriction Enzyme-Mediated Integration (REMI) Mutagenesis -- RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium -- Imaging and Localization Methods -- Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies -- Investigating Gene Expression -- Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging -- Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells -- Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development -- Under-Agarose Chemotaxis of Dictyostelium discoideum -- Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells -- Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum -- Dictyostelium as Model Organism -- Analysis of Signal Transduction -- Assaying Chemotaxis of Dictyostelium Cells -- Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering -- Quantitative and Microscopic Methods for Studying the Endocytic Pathway -- Preparation of Intact, Highly Purified Phagosomes from Dictyostelium -- Assaying Cell-Cell Adhesion -- Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells -- An Improved Method for Dictyostelium Centrosome Isolation -- Epiggenetics in Dictyostelium -- Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions -- Pharmacogenetics -- How to Assess and Study Cell Death in Dictyostelium discoideum.
520			_aDictyostelium discoideum Protocols presents a comprehensive collection of cutting-edge molecular biological protocols for studying D. discoideum, with emphasis on its utility in the study of fundamental cellular processes including signal transduction, chemotaxis, cell motility, cytokinesis, phagocytosis, and aspects of development such as cell sorting, pattern formation, and cell type differentiation. Written by experts in the field, this important guide provides easy access to current methods and techniques for the study of D. discoideum. For the newcomer to the field, it offers a complete and inclusive introduction to this important model organism. The book is divided into four main parts, which include an introduction to the organism that provides integral community resources and genome-wide approaches; basic methods and available molecular genetic techniques for study of the organism; imaging and localization methods; and a discussion of D. discoideum, with emphasis on its unique advantages as a model system. This in-depth guide provides a complete introduction to and thorough handbook for the study of D. discoideum, including a discussion of the implications of the recently completed Dictyostelium genome project in strengthening the position of D. discoideum as a model organism for studying fundamental cell processes and aspects of development. Researchers at all levels of experience will benefit from the presentations throughout of the most current, useful, and innovative techniques for the study of D. discoideum from leading Dictyostelium scientists.
988			_aSpringer_Protocols_2006
650		7	_2embne _9138683 _aHongos
776	0	8	_iPrinted edition: _z9781617376771
776	0	8	_iPrinted edition: _z9781588296238
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597451444 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zPRE