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007			cr nn 008mamaa
008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592590681
024	7		_a10.1385/1592590683 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH467 _b2000 EB
245	0	0	_aDNA Repair Protocols : _bProkaryotic Systems _cedited by Pat Vaughan
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (X, 192 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v152
505	0		_aAssays To Detect And Quantitate DNA Repair Activity -- Repair of A/G and A/8-oxoG Mismatches by MutY Adenine DNA Glycosylase -- Assays for the Repair of Oxidative Damage by Formamidopyrimidine Glycosylase (Fpg) and 8-)Oxoguanine DNA Glycosylase (OGG-1) -- Detection and Quantitation of Uracil DNA Glycosylase Activity -- Detection of DNA Deoxyribophosphodiesterase Activity -- O 6-Alkylguanine-DNA Alkytransferase Assay -- The E. coli Vsr Endonuclease -- Coupling of DNA Helicase Function to DNA Strand Exchange Activity -- Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity -- Holliday Junction Branch Migration and Resolution -- MutS-DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance -- Use Of DNA Repair Enzymes As Tools In Molecular Biology -- Flexible Genetic Engineering Using RecA Protein -- Interspecies Recombination and Mismatch Repair -- The Use of Immobilized Mismatch Binding Protein in Mutation/SNP Detection -- Use of Uracil DNA Glycosylase in the Detection of Known DNA Mutations and Polymorphisms -- Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms -- The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection.
520			_aDNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.
988			_aSpringer_Protocols_2000
650		7	_2embne _9301511 _aADN _xReplicación _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617371127
776	0	8	_iPrinted edition: _z9780896036437
776	0	8	_iPrinted edition: _z9781489941909
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590683 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI