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008			130109s2013 xxua o |||| 0|eng d
020			_a9781627032605
024	7		_a10.1007/978-1-62703-260-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623.5 .M47 _b2013 EB
245	0	0	_aSynthetic Messenger RNA and Cell Metabolism Modulation : _bMethods and Protocols _cedited by Peter M. Rabinovich
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 324 páginas) _b49 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v969
505	0		_aCell Engineering with Synthetic Messenger RNA -- In vitro Transcription of Long RNA Containing Modified Nucleosides -- HPLC Purification of in vitro Transcribed Long RNA -- Synthetic mRNAs with Superior Translation and Stability Properties -- Non-Viral, Cationic Lipid-Mediated Delivery of mRNA -- Light-Induced mRNA Transfection -- Engineering B Cells with mRNA -- Programming Human Dendritic Cells with mRNA -- Large Volume Flow Electroporation of mRNA: Clinical Scale Process -- Retrovirus-Based mRNA Transfer for Transient Cell Manipulation -- mRNA Delivery to Human Dendritic Cells by Recombinant -- Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy -- Natural Killer Cell Reprogramming with Chimeric Immune Receptors -- Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA -- Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies -- Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA -- Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA -- mRNA electroporation as a Tool for Immunomonitoring -- mRNA PCR-Based Epitope Chase Method.
520			_aSynthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.
988			_aSpringer_Protocols_2013
650		7	_2embne _9144940 _aARN _vManuales de laboratorio
650		7	_2embne _9138638 _aMetabolismo _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627032612
776	0	8	_iPrinted edition: _z9781627032599
776	0	8	_iPrinted edition: _z9781493959679
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-260-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI