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008			100301s2005 xxu| o |||| 0|eng d
020			_a9781592598489
024	7		_a10.1385/159259848X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR376 _b2005 EB
245	0	0	_aDNA Viruses : _bMethods and Protocols _cedited by Paul M. Lieberman
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XIV, 498 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v292
505	0		_aViral Detection -- Viral Detection -- Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1 -- Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR -- Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo -- Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo -- Reporter Cell Lines for the Detection of Herpes Simplex Viruses -- Virus Structure And Imaging -- Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy -- Studying the Structure of Large Viruses With Multiresolution Imaging -- Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence -- Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy -- FTIR Microscopy Detection of Cells Infected With Viruses -- Virus Entry -- The JC Virus-Like Particle Overlay Assay -- Analysis of Fusion Using a Virus-Free Cell Fusion Assay -- Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells -- Gene Expression -- Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence -- Identification and Characterization of Herpesviral Immediate-Early Genes -- Replication And Genome Maintenance -- Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids -- DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins -- Pathogenesis -- Pre-B-Cell Colony Formation Assay -- Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis -- Complex Cell Systems -- Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection -- Human Papillomavirus Type 31 Life Cycle Methods for Study Using Tissue Culture Models -- Recombinant Genetics -- Molecular Genetics of Herpesviruses A Recombinant Technology Approach -- Molecular Genetics of DNA Viruses Recombinant Virus Technology -- Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis -- Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology -- Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs -- Computation/Systems Biology Of Viruses -- Design of a Herpes Simplex Virus Type 2 Long Oligonucleotide-Based Microarray -- Real-Time Quantitative PCR Analysis of Viral Transcription -- Rapid Screening of Chemical Inhibitors That Block Processive DNA Synthesis of Herpesviruses.
520			_aRecently, modern laboratory techniques have been applied with great success to DNA viruses, an important viral class that shares basic survival strategies and mechanisms of pathogenesis, and also provides model systems for the investigation of basic biological processes. In DNA Viruses: Methods and Protocols, a panel of expert academic and biomedical researchers describe in detail the innovative methods they have developed to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in research virology with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
988			_aSpringer_Protocols_2005
650		7	_2embne _9413113 _aVirología _vManuales de laboratorio
650		7	_2embne _9140806 _aADN _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781588293534
776	0	8	_iPrinted edition: _z9781627038102
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259848X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI