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008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592596713
024	7		_a10.1385/1592596711 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP606 .P76 _b2004 EB
245	0	0	_aMAP Kinase Signaling Protocols : _cedited by Rony Seger
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 332 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v250
505	0		_aThe ERK Cascade As a Prototype of MAPK Signaling Pathways -- Determination of ERK Activity -- Detection of ERK1/2 Activities Using Affinity Reagents -- Activation of SAPKs/JNKs and p38s In Vitro -- Investigating the Cellular BMK1/ERK5 Signaling Pathway -- Pull-Down Assays for Guanosine 5?-Triphosphate-Bound Ras-Like Guanosine 5?-Triphosphatases -- Regulation of MAPK Cascades by Protein Tyrosine Phosphatases -- Use of Inhibitors in the Study of MAPK Signaling -- Structure of MAPKs -- Subcellular Localization of MAPKs -- Study of MAPK Signaling Using Knockout Mice -- Computer Simulation of MAPK Signal Transduction -- Signaling by Growth Factor Receptors -- Activation of MAPKs by G Protein-Coupled Receptors -- Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation -- Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries -- Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells -- Methods in Functional Proteomics -- Practical Methods for Deuterium Exchange/Mass Spectrometry -- Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling -- MAPK Cascades in the Brain.
520			_aMitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.
988			_aSpringer_Protocols_2004
650		7	_2embne _9510854 _aProteína cinasas _xInvestigación
776	0	8	_iPrinted edition: _z9781617372957
776	0	8	_iPrinted edition: _z9780896039988
776	0	8	_iPrinted edition: _z9781489939777
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596711 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI