000			04028nam a2200409 i 4500
999			_c392195 _d392195
001			392195
003			ES-MaUEC
005			20231024095051.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			171213s2018 xxua o |||| 0|eng d
020			_a9781493975402
024	7		_a10.1007/978-1-4939-7540-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623.5 .M47 _b2018 EB
245	0	0	_amRNA Decay : _bMethods and Protocols _cedited by Shireen R. Lamandé
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 227 páginas) _b34 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1720
505	0		_a5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives -- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq) -- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay -- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH -- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins -- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs -- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos -- Integration of ENCODE RNAseq and eCLIP Data Sets -- Identifying miRNA Targets Using AgoRIP-Seq.- Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets -- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry -- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay -- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq) -- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity -- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.- Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.
520			_aThis detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.
988			_aSpringer_Protocols_2018
650		7	_2embne _9144940 _aARN _vManuales de laboratorio
650		7	_2embne _9138638 _aMetabolismo _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493975396
776	0	8	_iPrinted edition: _z9781493975419
776	0	8	_iPrinted edition: _z9781493985159
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7540-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI