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008			171006s2018 xxu| o |||| 0|eng d
020			_a9781493974016
024	7		_a10.1007/978-1-4939-7401-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC269.7 _b2018 EB
245	0	0	_aCancer Stem Cells : _bMethods and Protocols _cedited by Gianpaolo Papaccio, Vincenzo Desiderio
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 223 páginas) _b30 ilustraciones, 13 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1692
505	0		_aIntroduction to Cancer Stem Cells: Past, Present, and Future -- Surface Markers for the Identification of Cancer Stem Cells -- The Role of CD44 and Cancer Stem Cells -- Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay -- Isolation of Cancer Stem Cells by Side Population Method -- Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres -- In Vitro Tumorigenic Assay: The Tumor Spheres Assay -- In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells -- Xenograft as In Vivo Experimental Model -- How to Assess Drug Resistance in Cancer Stem Cells -- Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells -- Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) -- Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System -- Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform -- Assessing DNA Methylation in Cancer Stem Cells -- Histones Acetylation and Cancer Stem Cells (CSCs) -- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods -- Circulating Tumor Cells.
520			_aThis detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.  Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. .
988			_aSpringer_Protocols_2018
650		7	_2embne _9146156 _aCélulas cancerosas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493974009
776	0	8	_iPrinted edition: _z9781493974023
776	0	8	_iPrinted edition: _z9781493984749
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7401-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _eb _zSI