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020 _a9781493973804
024 7 _a10.1007/978-1-4939-7380-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH599
_b2018 EB
245 0 0 _aChromatin Immunoprecipitation :
_bMethods and Protocols
_cedited by Neus Visa, Antonio Jordán-Pla
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (X, 243 páginas)
_b35 ilustraciones, 26 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1689
505 0 _aChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance -- Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments -- How to Combine ChIP with qPCR -- Analysis of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-Chip) -- Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next Generation Sequencing -- Chromatin RNA Immunoprecipitation (ChRIP) -- DNA Accessibility by MNase Digestions -- Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-Seq) -- ChIP-re-ChIP: Co-Occupancy Analysis by Sequential Chromatin Immunoprecipitation -- Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging -- Chromatin Immunoprecipitation of Skeletal Muscle Tissue -- Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius.- Using Intra-ChIP to Measure Protein-DNA Interactions in Intracellular Pathogens -- Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers -- MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation -- Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis -- Analysis of ChIP-Seq Data in R/Bioconductor -- Spike-In Normalization of ChIP Data Using DNA-DIG-Antibody Complex.
520 _aThis up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas. The collection also includes protocols designed to improve the performance of ChIP for specific applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols features techniques, including bioinformatic analysis of ChIP data, will be of interest to a very broad research community in the fields of biochemistry, molecular biology, microbiology, and biomedicine.
988 _aSpringer_Protocols_2018
650 7 _2embne
_9144121
_aCromatina
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493973798
776 0 8 _iPrinted edition:
_z9781493973811
776 0 8 _iPrinted edition:
_z9781493984671
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7380-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_eb
_zSI